Anti-NCoA-2, clone 1C6.3

Code: MABS1688 D2-231

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected nuclear NCoA-2 immunoreactivity in human lymphoma, ovarian cancer, and small int...


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Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected nuclear NCoA-2 immunoreactivity in human lymphoma, ovarian cancer, and small intestine tissue sections.

Detect NCOA2 using this mouse monoclonal Anti-NCoA-2 Antibody, clone 1C6.3, Cat. No. MABS1688, validated for use in Immunohistochemistry (Paraffin) and Western blotting.

General description

Nuclear receptor coactivator 2 (UniProt Q15596; also known as bHLHe75, Class E basic helix-loop-helix protein 75, hTIF2, NCoA-2, Transcriptional intermediary factor 2) is encoded by the NCOA2 (also known as BHLHE75, SRC2, TIF2) gene (Gene ID 10499) in human. Nuclear receptor coactivator 2 (NCoA-2) is a 160 kDa transcriptional coactivator for steroid receptors and nuclear receptors that acts as a coactivator of the steroid binding domain (AF-2), but not the modulating N-terminal domain (AF-1). It possesses a single NR interaction domain (NID) and two autonomous activation domains, AD1 and AD2. NCoA-2 assists nuclear receptors in the upregulation of DNA expression. In cells ligand activated nuclear receptors recruit NCoA-2 to DNA where it acylates histones to make targeted regions accessible to transcriptional machinery. Along with NCOA1 it controls energy balance between white and brown adipose tissues and also acts as a critical regulator of glucose metabolism. It is also involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. NCoA-2 is reported to positively regulate the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer. Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias.

Ref.: Voegel, JJ et al. (1998). EMBO J. 17, 507 519.

Immunogen

GST-tagged recombinant human NCoA-2 C-terminal region fragment..

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in human osteosarcoma cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NCoA-2 in 10 µg of U2OS human osteosarcoma cell lysate.

Specificity

Clone 1C6.3 targeted epitope lies within the C-terminal one fourth region of the full-length human NCoA-2.

Target description

~160 kDa observed. 159.0 kDa (Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
clone1C6.3, monoclonal
Gene Informationhuman ... NCOA2(10499)
isotypeIgG2aκ
NCBI accession no.NP_059111
Quality Level100
shipped inwet ice
species reactivityhuman
technique(s)western blot: suitable, immunohistochemistry: suitable (paraffin)
UniProt accession no.Q15596
This product has met the following criteria to qualify for the following awards:



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